Target：Paxillin

Fields：Chemokine signaling pathway;VEGF signaling pathway;Focal adhesion;Leukocyte transendothelial migration;Regulation of actin cytoskeleton;Bacterial invasion of epithelial cells;Shigellosis;Yersinia infection;Human cytomegalovirus infection;Human papillomavirus infection;Human immunodeficiency virus 1 infection;Viral carcinogenesis;Proteoglycans in cancer

Gene Name：PXN

Protein Name：Paxillin

Human Gene Id：5829

Human Swiss Prot No：P49023

Mouse Gene Id：19303

Mouse Swiss Prot No：Q8VI36

Rat Gene Id：360820

Rat Swiss Prot No：Q66H76

Immunogen：Synthesized phospho-peptide around the phosphorylation site of human Paxillin (phospho Ser178)

Specificity：Phospho-Paxillin (S178) Polyclonal Antibody detects endogenous levels of Paxillin protein only when phosphorylated at S178.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PXN;Paxillin

Observed Band(KD)：65kD

Background： This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011],

Function：function:Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).,PTM:Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.,similarity:Belongs to the paxillin family.,similarity:Contains 3 LIM zinc-binding domains.,similarity:Contains 4 LIM zinc-binding domains.,subunit:Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds ASAP2. Int

Subcellular Location：Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389). .

Expression： Brain,Epithelium,Lung,Placenta,T-cell,Uterus,