Target：PC-PLD1
Fields：Glycerophospholipid metabolism;Ether lipid metabolism;Metabolic pathways;Ras signaling pathway;cAMP signaling pathway;Sphingolipid signaling pathway;Phospholipase D signaling pathway;Endocytosis;Fc gamma R-mediated phagocytosis;Glutamatergic synapse;GnRH signaling pathway;Parathyroid hormone synthesis, secretion and action;Pathways in cancer;Chemical carcinogenesis - reactive oxygen species;Pancreatic cancer;Choline metabolism in cancer
Gene Name：PLD1
Protein Name：Phospholipase D1
Human Gene Id：5337
Human Swiss Prot No：Q13393
Mouse Swiss Prot No：Q9Z280
Rat Gene Id：25096
Rat Swiss Prot No：P70496
Immunogen：The antiserum was produced against synthesized peptide derived from human PLD1 around the phosphorylation site of Ser561. AA range:527-576
Specificity：Phospho-PC-PLD1 (S561) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at S561.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：PLD1;Phospholipase D1;PLD 1;hPLD1;Choline phosphatase 1;Phosphatidylcholine-hydrolyzing phospholipase D1
Molecular Weight(Da)：124kD
Background： This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011],
Function：catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,enzyme regulation:Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42. Inhibited by oleate.,function:Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.,online information:Phospholipase D entry,similarity:Belongs to the phospholipase D family.,similarity:Contains 1 PH domain.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 PLD phosphodiesterase domains.,subunit:Interacts with PIP5K1A.,tissue specificity:Expressed abundant
Subcellular Location：Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Late endosome membrane ; Lipid-anchor ; Cytoplasmic side .
Expression：Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
