Target：PFK-2 car

Fields：Fructose and mannose metabolism;Metabolic pathways;AMPK signaling pathway;Thyroid hormone signaling pathway

Gene Name：PFKFB2

Protein Name：6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

Human Gene Id：5208

Human Swiss Prot No：O60825

Mouse Gene Id：18640

Mouse Swiss Prot No：P70265

Rat Gene Id：24640

Rat Swiss Prot No：Q9JJH5

Immunogen：The antiserum was produced against synthesized peptide derived from human PFKFB2 around the phosphorylation site of Ser483. AA range:451-500

Specificity：Phospho-PFK-2 car (S483) Polyclonal Antibody detects endogenous levels of PFK-2 car protein only when phosphorylated at S483.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PFKFB2;6-phosphofructo-2-kinase/fructose-2;6-bisphosphatase 2;6PF-2-K/Fru-2,6-P2ase 2;PFK/FBPase 2;6PF-2-K/Fru-2,6-P2ase heart-type isozyme

Observed Band(KD)：58kD

Background： The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.,catalytic activity:Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate.,enzyme regulation:Phosphorylation results in the activation of the kinase activity.,function:Synthesis and degradation of fructose 2,6-bisphosphate.,similarity:In the C-terminal section; belongs to the phosphoglycerate mutase family.,subunit:Homodimer.,tissue specificity:Heart.,

Subcellular Location：cytosol,

Expression：Heart.