Target：PIP5K

Fields：Inositol phosphate metabolism;Metabolic pathways;Phosphatidylinositol signaling system;Phagosome;Regulation of actin cytoskeleton

Gene Name：PIKFYVE

Protein Name：1-phosphatidylinositol 3-phosphate 5-kinase

Human Gene Id：200576

Human Swiss Prot No：Q9Y2I7

Mouse Gene Id：18711

Mouse Swiss Prot No：Q9Z1T6

Immunogen：The antiserum was produced against synthesized peptide derived from human PIP5K around the phosphorylation site of Ser307. AA range:273-322

Specificity：Phospho-PIP5KIII (S307) Polyclonal Antibody detects endogenous levels of PIP5KIII protein only when phosphorylated at S307.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PIKFYVE;KIAA0981;PIP5K3;1-phosphatidylinositol 3-phosphate 5-kinase;Phosphatidylinositol 3-phosphate 5-kinase;FYVE finger-containing phosphoinositide kinase;PIKfyve;Phosphatidylinositol 3-phosphate 5-kinase type III;PIPkin-III;Type

Molecular Weight(Da)：237kD

Background： Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKf

Function：catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.,disease:Defects in PIKFYVE are the cause of corneal fleck dystrophy (CFD) [MIM:121850]. CFD is an autosomal dominant disorder of the cornea characterized by numerous small white flecks scattered in all levels of the stroma. Although CFD may occasionally cause mild photophobia, patients are typically asymptomatic and have normal vision.,function:Supports the intracellular PIP pool and to a lesser extent, the PI 4,5-P(2) pool. It generates PIP from PI and, to a lesser extent, PI 4,5-P(2) from PI 4-P. There are indications that it phosphorylates the D-5 rather than the D-4 position. Has a role in endosome-related membrane trafficking.,similarity:Contains 1 DEP domain.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 PI5K domain.,subcellular location:

Subcellular Location：Endosome membrane ; Peripheral membrane protein . Early endosome membrane ; Peripheral membrane protein. Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein . Late endosome membrane ; Peripheral membrane protein . Mainly associated with membranes of the late endocytic pathway. .

Expression： Brain,Epithelium,PCR rescued clones,T-cell,