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FoxO1 (phospho Ser256) Polyclonal Antibody
FoxO1 (phospho Ser256) Polyclonal Antibody
FoxO1 (phospho Ser256) Polyclonal Antibody
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商品属性

Target:FoxO1

Fields:FoxO signaling pathway;AMPK signaling pathway;Longevity regulating pathway;Longevity regulating pathway - multiple species;Cellular senescence;Insulin signaling pathway;Thyroid hormone signaling pathway;Glucagon signaling pathway;Insulin resistance;AGE-RAGE signaling pathway in diabetic complications;Alcoholic liver disease;Shigellosis;Human papillomavirus infection;Pathways in cancer;Transcriptional misregulation in cancer;Prostate cancer

Gene Name:FOXO1

Protein Name:Forkhead box protein O1

Human Gene Id:2308

Human Swiss Prot No:Q12778

Mouse Gene Id:56458

Mouse Swiss Prot No:Q9R1E0

Rat Gene Id:84482

Rat Swiss Prot No:G3V7R4

Immunogen:The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272

Specificity:Phospho-FoxO1 (S256) Polyclonal Antibody detects endogenous levels of FoxO1 protein only when phosphorylated at S256.

Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source:Polyclonal, Rabbit,IgG

Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration:1 mg/ml

Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name:FOXO1;FKHR;FOXO1A;Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma

Observed Band(KD):82kD

Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],

Function:disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.,function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles betw

Subcellular Location:Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation

Expression:Ubiquitous.

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