Target：ZAP-70
Fields：Ras signaling pathway;NF-kappa B signaling pathway;Natural killer cell mediated cytotoxicity;Th1 and Th2 cell differentiation;Th17 cell differentiation;T cell receptor signaling pathway;Yersinia infection;PD-L1 expression and PD-1 checkpoint pathway in cancer;Primary immunodeficiency
Gene Name：ZAP70
Protein Name：Tyrosine-protein kinase ZAP-70
Human Gene Id：7535
Human Swiss Prot No：P43403
Mouse Gene Id：22637
Mouse Swiss Prot No：P43404
Immunogen：The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr319. AA range:286-335
Specificity：Phospho-ZAP-70 (Y319) Polyclonal Antibody detects endogenous levels of ZAP-70 protein only when phosphorylated at Y319.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：ZAP70;SRK;Tyrosine-protein kinase ZAP-70;70 kDa zeta-chain associated protein;Syk-related tyrosine kinase
Observed Band(KD)：70kD
Background： This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.,domain:The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.,function:Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.,online information:ZAP70 mutation db,PTM:Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.,similarity:Contains 1 prote
Subcellular Location：Cytoplasm . Cell membrane ; Peripheral membrane protein . In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). .
Expression：Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
