Target：14-3-3 ζ

Fields：Cell cycle;Oocyte meiosis;PI3K-Akt signaling pathway;Hippo signaling pathway;Hepatitis C;Hepatitis B;Viral carcinogenesis

Gene Name：YWHAZ

Protein Name：14-3-3 protein zeta/delta

Human Gene Id：7534

Human Swiss Prot No：P63104

Mouse Gene Id：22631

Mouse Swiss Prot No：P63101

Rat Gene Id：25578

Rat Swiss Prot No：P63102

Immunogen：The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. AA range:24-73

Specificity：Phospho-14-3-3 ζ (S58) Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein only when phosphorylated at S58.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：YWHAZ;14-3-3 protein zeta/delta;Protein kinase C inhibitor protein 1;KCIP-1

Observed Band(KD)：30kD

Background： This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],

Function：caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to

Subcellular Location：Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.

Expression： B-cell lymphoma,Bone marrow