Target：IKKα/β

Fields：Antifolate resistance;MAPK signaling pathway;Ras signaling pathway;Chemokine signaling pathway;NF-kappa B signaling pathway;FoxO signaling pathway;mTOR signaling pathway;PI3K-Akt signaling pathway;Apoptosis;Osteoclast differentiation;Toll-like receptor signaling pathway;NOD-like receptor signaling pathway;RIG-I-like receptor signaling pathway;Cytosolic DNA-sensing pathway;C-type lectin receptor signaling pathway;IL-17 signaling pathway;Th1 and Th2 cell differentiation;Th17 cell differentiation;T cell receptor signaling pathway;B cell receptor signaling pathway;TNF signaling pathway;Adipocytokine signaling pathway;Alcoholic liver disease;Alzheimer disease;Epithelial cell signaling in Helicobacter pylori infection;Pathogenic Escherichia coli infection;Shigellosis;Salmonella infection;Yersinia infection;Chagas disease;Toxoplasmosis;Hepatitis C;Hepatitis B;Measles;Human cytomegalovirus infection;Influenza A;Human pap

Gene Name：CHUK/IKBKB

Protein Name：Inhibitor of nuclear factor kappa-B kinase subunit alpha

Human Gene Id：1147/3551

Human Swiss Prot No：O15111/O14920

Mouse Gene Id：16150

Rat Gene Id：84351

Rat Swiss Prot No：Q9QY78

Immunogen：The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta around the phosphorylation site of Ser180/181. AA range:151-200

Specificity：Phospho-IKKα/β (S180/181) Polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at S180/181.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CHUK;IKKA;TCF16;Inhibitor of nuclear factor kappa-B kinase subunit alpha;I-kappa-B kinase alpha;IKK-A;IKK-alpha;IkBKA;IkappaB kinase;Conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;IKK1;Nuclear factor NF-kappa-B

Observed Band(KD)：85kD

Background： This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK

Subcellular Location：Cytoplasm . Nucleus . Shuttles between the cytoplasm and the nucleus.

Expression：Widely expressed.