Target：Syntaxin 1
Fields：SNARE interactions in vesicular transport;Synaptic vesicle cycle;Insulin secretion;Huntington disease;Pathways of neurodegeneration - multiple diseases;Amphetamine addiction
Gene Name：STX1A
Protein Name：Syntaxin-1A
Human Gene Id：6804
Human Swiss Prot No：Q16623
Mouse Gene Id：20907
Mouse Swiss Prot No：O35526
Rat Gene Id：116470
Rat Swiss Prot No：P32851
Immunogen：The antiserum was produced against synthesized peptide derived from human Syntaxin 1A around the phosphorylation site of Ser14. AA range:1-50
Specificity：Phospho-Syntaxin 1 (S14) Polyclonal Antibody detects endogenous levels of Syntaxin 1 protein only when phosphorylated at S14.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：STX1A;STX1;Syntaxin-1A;Neuron-specific antigen HPC-1
Observed Band(KD)：35kD
Background： This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],
Function：disease:Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,function:Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis.,similarity:Belongs to the syntaxin family.,similarity:Contains 1 t-SNARE coiled-coil homology domain.,subunit:Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with OTOF (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU.,tissue specificity:Isoform 1 is highly expressed in embryonic spinal chord and ganglia
Subcellular Location：Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome . Cell membrane . Colocalizes with KCNB1 at the cell membrane. .; [Isoform 2]: Secreted .
Expression：[Isoform 1]: Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex. ; [Isoform 2]: Expressed in heart, liver, fat, skeletal muscle, kidney and brain.
