Target：Smad5

Fields：TGF-beta signaling pathway;Signaling pathways regulating pluripotency of stem cells

Gene Name：SMAD5

Protein Name：Mothers against decapentaplegic homolog 5

Human Gene Id：4090

Human Swiss Prot No：Q99717

Mouse Swiss Prot No：P97454

Rat Gene Id：59328

Rat Swiss Prot No：Q9R1V3

Immunogen：Synthesized peptide derived from the Internal region of human Smad5.

Specificity：Smad5 Polyclonal Antibody detects endogenous levels of Smad5 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：SMAD5;MADH5;Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5

Observed Band(KD)：52kD

Background： The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],

Function：function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2.,tissue specificity:Ubiquitous.,

Subcellular Location：Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

Expression：Ubiquitous.