Target：SERCA1

Fields：Calcium signaling pathway;cGMP-PKG signaling pathway;cAMP signaling pathway;Cardiac muscle contraction;Adrenergic signaling in cardiomyocytes;Thyroid hormone signaling pathway;Pancreatic secretion;Alzheimer disease;Spinocerebellar ataxia;Pathways of neurodegeneration - multiple diseases;Hypertrophic cardiomyopathy;Arrhythmogenic right ventricular cardiomyopathy;Dilated cardiomyopathy;Diabetic cardiomyopathy

Gene Name：ATP2A1

Protein Name：Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Human Gene Id：487

Human Swiss Prot No：O14983

Mouse Gene Id：11937

Mouse Swiss Prot No：Q8R429

Rat Gene Id：116601

Rat Swiss Prot No：Q64578

Immunogen：The antiserum was produced against synthesized peptide derived from human ATP2A1. AA range:548-597

Specificity：SERCA1 Polyclonal Antibody detects endogenous levels of SERCA1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ATP2A1;Sarcoplasmic/endoplasmic reticulum calcium ATPase 1;SERCA1;SR Ca(2+)-ATPase 1;Calcium pump 1;Calcium-transporting ATPase sarcoplasmic reticulum type; fast twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca(2+) AT

Observed Band(KD)：100kD

Background： This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013],

Function：catalytic activity:ATP + H(2)O + Ca(2+)(Cis) = ADP + phosphate + Ca(2+)(Trans).,developmental stage:Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult, while isoform SERCA1B predominates in neo-natal fibers.,disease:Defects in ATP2A1 are the cause of Brody disease (BD) [MIM:601003]. BD is an autosomal recessive myopathy characterized by increasing impairment of relaxation of fast twist skeletal muscle during exercise.,enzyme regulation:Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN.,function:This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved

Subcellular Location：Endoplasmic reticulum membrane ; Multi-pass membrane protein . Sarcoplasmic reticulum membrane ; Multi-pass membrane protein .

Expression：Skeletal muscle, fast twitch muscle (type II) fibers.