Target：Cdc34
Fields：Ubiquitin mediated proteolysis
Gene Name：CDC34
Protein Name：Ubiquitin-conjugating enzyme E2 R1
Human Gene Id：997
Human Swiss Prot No：P49427
Mouse Gene Id：216150
Mouse Swiss Prot No：Q8CFI2
Immunogen：The antiserum was produced against synthesized peptide derived from the Internal region of human CDC34. AA range:121-170
Specificity：Cdc34 Polyclonal Antibody detects endogenous levels of Cdc34 protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：CDC34;UBCH3;UBE2R1;Ubiquitin-conjugating enzyme E2 R1;Ubiquitin-conjugating enzyme E2-32 kDa complementing;Ubiquitin-conjugating enzyme E2-CDC34;Ubiquitin-protein ligase R1
Observed Band(KD)：34kD
Background： The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC).,
Subcellular Location：Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.
Expression：Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
