Target：Cdc25B

Fields：MAPK signaling pathway;Cell cycle;Progesterone-mediated oocyte maturation;MicroRNAs in cancer

Gene Name：CDC25B

Protein Name：M-phase inducer phosphatase 2

Human Gene Id：994

Human Swiss Prot No：P30305

Mouse Gene Id：12531

Mouse Swiss Prot No：P30306

Rat Gene Id：171103

Rat Swiss Prot No：P48966

Immunogen：The antiserum was produced against synthesized peptide derived from human CDC25B. AA range:289-338

Specificity：Cdc25B Polyclonal Antibody detects endogenous levels of Cdc25B protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CDC25B;CDC25HU2;M-phase inducer phosphatase 2;Dual specificity phosphatase Cdc25B

Observed Band(KD)：65kD

Background：cell division cycle 25B(CDC25B) Homo sapiens CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008],

Function：catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,enzyme regulation:Stimulated by B-type cyclins.,function:Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.,PTM:Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,

Subcellular Location：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .

Expression： Brain,Rectum tumor,