Target：Epsin 1

Fields：Endocytosis

Gene Name：EPN1

Protein Name：Epsin-1

Human Gene Id：29924

Human Swiss Prot No：Q9Y6I3

Mouse Gene Id：13854

Mouse Swiss Prot No：Q80VP1

Rat Gene Id：117277

Rat Swiss Prot No：O88339

Immunogen：Synthesized peptide derived from Epsin 1 . at AA range: 140-220

Specificity：Epsin 1 Polyclonal Antibody detects endogenous levels of Epsin 1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：EPN1;Epsin-1;EH domain-binding mitotic phosphoprotein;EPS-15-interacting protein 1

Observed Band(KD)：85kD

Background： This gene encodes a member of the epsin protein family. The encoded protein binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Loss of function of this gene is associated with reduced tumor growth and progression in certain cancer types. [provided by RefSeq, Mar 2016],

Function：domain:The DPW repeat domain is involved in AP2A2 and clathrin binding.,domain:The NPF repeat domain is involved in EPS15 binding.,function:Binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.,online information:The bubble's bend -Issue 42 of January 2004,PTM:Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.,PTM:Ubiquitinated.,similarity:Belongs to the epsin family.,similarity:Contains 1 ENTH (epsin N-terminal homology) domain.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,subcellular location:Associated with the cytoplasmic membrane

Subcellular Location：Cytoplasm . Cell membrane ; Peripheral membrane protein . Nucleus . Membrane, clathrin-coated pit . Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity). .

Expression： Brain,Epithelium,Mammary gland,