Target：CYP17A1

Fields：Steroid hormone biosynthesis;Metabolic pathways;Ovarian steroidogenesis;Prolactin signaling pathway;Cortisol synthesis and secretion;Cushing syndrome

Gene Name：CYP17A1

Protein Name：Steroid 17-alpha-hydroxylase/17,20 lyase

Human Gene Id：1586

Human Swiss Prot No：P05093

Mouse Swiss Prot No：P27786

Immunogen：The antiserum was produced against synthesized peptide derived from human Cytochrome P450 17A1. AA range:221-270

Specificity：CYP17A1 Polyclonal Antibody detects endogenous levels of CYP17A1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CYP17A1;CYP17;S17AH;Steroid 17-alpha-hydroxylase/17;20 lyase;CYPXVII;Cytochrome P450 17A1;Cytochrome P450-C17;Cytochrome P450c17;Steroid 17-alpha-monooxygenase

Observed Band(KD)：50kD

Background：cytochrome P450 family 17 subfamily A member 1(CYP17A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008],

Function：catalytic activity:A steroid + AH(2) + O(2) = a 17-alpha-hydroxysteroid + A + H(2)O.,cofactor:Heme group.,disease:Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).,enzyme regulation:Regulated predominantly by intracellular cAMP levels.,function:Conversion of pregnenolone and p

Subcellular Location：Endoplasmic reticulum membrane . Microsome membrane .

Expression： Brain,Corpus callosum,