Target：MYLIP

Fields：Cholesterol metabolism

Gene Name：MYLIP

Protein Name：E3 ubiquitin-protein ligase MYLIP

Human Gene Id：29116

Human Swiss Prot No：Q8WY64

Mouse Gene Id：218203

Mouse Swiss Prot No：Q8BM54

Rat Gene Id：306825

Rat Swiss Prot No：D3ZDI6

Immunogen：The antiserum was produced against synthesized peptide derived from human MYLIP. AA range:161-210

Specificity：MYLIP Polyclonal Antibody detects endogenous levels of MYLIP protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MYLIP;BZF1;IDOL;BM-023;PP5242;E3 ubiquitin-protein ligase MYLIP;Inducible degrader of the LDL-receptor;Idol;Myosin regulatory light chain interacting protein;MIR

Observed Band(KD)：50kD

Background： The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008],

Function：developmental stage:Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.,domain:The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.,function:E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC). Stable or transient overexpression of MIR induces proteasomal degradation of MRLC inhibiting neurite outgrowth in presence of NGF. MIR counteracts the stabilization of MRLC by MIR-interacting saposin-like protein (MSAP/TMEM4) and reduces MSAP-stimulated neurite outgrowth.,pathway:Protein modification; protein ubiquitination.,PTM:Autoubiquitinated.,similarity:Contains 1 FERM domain.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with myosin regulatory light chain (MRLC) and TMEM4.,tissue specificity:Ubiquitously expressed.,

Subcellular Location：Cytoplasm . Cell membrane ; Peripheral membrane protein .

Expression：Ubiquitously expressed.