Target：MLTK

Fields：MAPK signaling pathway

Gene Name：MLTK

Protein Name：Mitogen-activated protein kinase kinase kinase MLT

Human Gene Id：51776

Human Swiss Prot No：Q9NYL2

Mouse Gene Id：65964

Mouse Swiss Prot No：Q9ESL4

Immunogen：The antiserum was produced against synthesized peptide derived from human MLTK. AA range:701-750

Specificity：MLTK Polyclonal Antibody detects endogenous levels of MLTK protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MLTK;ZAK;HCCS4;Mitogen-activated protein kinase kinase kinase MLT;Human cervical cancer suppressor gene 4 protein;HCCS-4;Leucine zipper- and sterile alpha motif-containing kinase;MLK-like mitogen-activated protein triple kinase;Mixe

Observed Band(KD)：91kD

Background： This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation by PKN1 and autophosphorylation on Thr-161 and Ser-165.,function:Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,subcellular location:Tr

Subcellular Location：Cytoplasm . Nucleus . Translocates to the nucleus upon ultraviolet B irradiation. .

Expression：Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.