Target:MLKL
Fields:Necroptosis;TNF signaling pathway;Salmonella infection
Gene Name:MLKL
Protein Name:Mixed lineage kinase domain-like protein
Human Gene Id:197259
Human Swiss Prot No:Q8NB16
Mouse Swiss Prot No:Q9D2Y4
Immunogen:Synthesized peptide derived from MLKL . at AA range: 40-120
Specificity:MLKL Polyclonal Antibody detects endogenous levels of MLKL protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000;IHC 1:50-300
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:MLKL;Mixed lineage kinase domain-like protein
Observed Band(KD):55kD
Background: This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],
Function:domain:The protein kinase domain is predicted to be catalytically inactive.,similarity:Belongs to the protein kinase superfamily.,similarity:Contains 1 protein kinase domain.,
Subcellular Location:Cytoplasm . Cell membrane . Nucleus . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671). Localizes to the nucleus in response to orthomyxoviruses infection (By similarity). .
Expression: Chondrocyte,Leukocyte,Lymph node,