Target：Pki α

Fields：Alcoholism

Gene Name：PKIA

Protein Name：cAMP-dependent protein kinase inhibitor alpha

Human Gene Id：5569

Human Swiss Prot No：P61925

Mouse Gene Id：18767

Mouse Swiss Prot No：P63248

Rat Gene Id：114906

Rat Swiss Prot No：P63249

Immunogen：The antiserum was produced against synthesized peptide derived from human IPKA. AA range:10-59

Specificity：Pki α Polyclonal Antibody detects endogenous levels of Pki α protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PKIA;PRKACN1;cAMP-dependent protein kinase inhibitor alpha;PKI-alpha;cAMP-dependent protein kinase inhibitor; muscle/brain isoform

Molecular Weight(Da)：8kD

Background： The protein encoded by this gene is a member of the cAMP-dependent protein kinase (PKA) inhibitor family. This protein was demonstrated to interact with and inhibit the activities of both C alpha and C beta catalytic subunits of the PKA. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008],

Function：function:Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.,miscellaneous:The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.,similarity:Belongs to the PKI family.,

Subcellular Location：nucleus,cytoplasm,

Expression： Skeletal muscle,