Target：NUMB

Fields：Notch signaling pathway

Gene Name：NUMB

Protein Name：Protein numb homolog

Human Gene Id：8650

Human Swiss Prot No：P49757

Mouse Gene Id：18222

Mouse Swiss Prot No：Q9QZS3

Rat Swiss Prot No：Q2LC84

Immunogen：Synthesized peptide derived from the Internal region of human NUMB.

Specificity：NUMB Polyclonal Antibody detects endogenous levels of NUMB protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000; IF ICC 1:100-500;ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NUMB;Protein numb homolog;h-Numb;Protein S171

Observed Band(KD)：70kD

Background： The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

Function：function:Implicated in the control of cell fate decisions during development.,PTM:Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.,sequence caution:Intron retention.,similarity:Contains 1 PID domain.,subunit:Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis.,

Subcellular Location：Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Localizes to perinuclear endosomes in an AAK1-dependent manner. .

Expression： B cells,Blood,Brain,Cervix carcinoma,Epithelium,Fetal brain,HeLa cells,Live