Target:RhoGEF p115
Fields:Vascular smooth muscle contraction;Platelet activation;Regulation of actin cytoskeleton;Parathyroid hormone synthesis, secretion and action;Pathogenic Escherichia coli infection;Yersinia infection;Human cytomegalovirus infection;Pathways in cancer;Proteoglycans in cancer;Lipid and atherosclerosis
Gene Name:ARHGEF1
Protein Name:Rho guanine nucleotide exchange factor 1
Human Gene Id:9138
Human Swiss Prot No:Q92888
Mouse Gene Id:16801
Mouse Swiss Prot No:Q61210
Rat Swiss Prot No:Q9Z1I6
Immunogen:The antiserum was produced against synthesized peptide derived from human ARHGEF1. AA range:162-211
Specificity:RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:ARHGEF1;Rho guanine nucleotide exchange factor 1;115 kDa guanine nucleotide exchange factor;p115-RhoGEF;p115RhoGEF;Sub1.5
Observed Band(KD):105kD
Background: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],
Function:domain:The DH domain is involved in interaction with CCPG1.,domain:The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.,function:Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12.,PTM:Phosphorylated by PKCA.,sequence caution:Contaminating sequence. Sequence of unknown origin in the N-terminal part.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane by activ
Subcellular Location:Cytoplasm . Membrane . Translocated to the membrane by activated GNA13 or LPA stimulation.
Expression:Ubiquitously expressed.