Target：P4K2A

Fields：Inositol phosphate metabolism;Metabolic pathways;Phosphatidylinositol signaling system

Gene Name：PI4K2A

Protein Name：Phosphatidylinositol 4-kinase type 2-alpha (EC 2.7.1.67) (Phosphatidylinositol 4-kinase type II-alpha)

Human Gene Id：55361

Human Swiss Prot No：Q9BTU6

Mouse Swiss Prot No：Q2TBE6

Rat Swiss Prot No：Q99M64

Immunogen：Synthesized peptide derived from human protein . at AA range: 50-130

Specificity：P4K2A Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：52kD

Background： Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008],

Function：catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.,function:Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells.,similarity:Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.,similarity:Contains 1 PI3K/PI4K domain.,subcellular location:Found in subdomains of the plasma membrane termed non-caveolar membrane rafts.,tissue specificity:Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed

Subcellular Location：Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor . Membrane raft . Cell projection, dendrite . Cell junction, synapse, presynaptic cell membrane . Cell junction, synapse, synaptosome . Mitochondrion . Endosome . Cytoplasmic vesicle . Membrane ; Lipid-anchor . Cell membrane . Perikaryon . Cell projection, neuron projection . Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. Transported from neuronal cell body to neuron projections and neurite tips in a BLOC-1- and AP-3-complexes-dependent manner. .

Expression：Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.