Target:RHG05
Fields:Focal adhesion;Leukocyte transendothelial migration
Gene Name:ARHGAP5 RHOGAP5
Protein Name:Rho GTPase-activating protein 5 (Rho-type GTPase-activating protein 5) (p190-B)
Human Gene Id:394
Human Swiss Prot No:Q13017
Mouse Swiss Prot No:P97393
Immunogen:Synthesized peptide derived from human protein . at AA range: 280-360
Specificity:RHG05 Polyclonal Antibody detects endogenous levels of protein.
Formulation:Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Observed Band(KD):165kD
Background: Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function:function:GTPase-activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of p120GAP.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 4 FF domains.,subcellular location:Also membrane-associated when found in fibrillar patterns that colocalize with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin.,tissue specificity:Expressed in kidney, brain, liver and lung.,
Subcellular Location:Cytoplasm . Cell membrane ; Peripheral membrane protein . Also membrane-associated in a fibrillar pattern that colocalizes with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin. .
Expression:Detected in skin fibroblasts (at protein level) (PubMed:8537347).