Target:PDCD4
Fields:Proteoglycans in cancer;MicroRNAs in cancer
Gene Name:PDCD4
Protein Name:Programmed cell death protein 4
Human Gene Id:27250
Human Swiss Prot No:Q53EL6
Mouse Gene Id:18569
Mouse Swiss Prot No:Q61823
Rat Gene Id:64031
Rat Swiss Prot No:Q9JID1
Immunogen:The antiserum was produced against synthesized peptide derived from human PDCD4. AA range:420-469
Specificity:Pdcd-4 Polyclonal Antibody detects endogenous levels of Pdcd-4 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:PDCD4;H731;Programmed cell death protein 4;Neoplastic transformation inhibitor protein;Nuclear antigen H731-like;Protein 197/15a
Observed Band(KD):51kD
Background: This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010],
Function:caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Loss of expression correlated with tumor progression of lung and colon carcinoma.,domain:Binds EIF4A1 via the MA3 domains.,function:Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Inhibits the helicase activity of EIF4A and cap-dependent translation. Binds RNA.,induction:IL2 stimulation inhibits expression, while IL12 increases expression.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the PDCD4 family.,similarity:Contains 2 MI domains.,subcellular location:Shuttles between the nucleus a
Subcellular Location:Nucleus . Cytoplasm . Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133). .
Expression:Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.