Target：PFK-2 liv

Fields：Fructose and mannose metabolism;Metabolic pathways;AMPK signaling pathway;Glucagon signaling pathway

Gene Name：PFKFB1

Protein Name：6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1

Human Gene Id：5207

Human Swiss Prot No：P16118

Mouse Gene Id：18639

Mouse Swiss Prot No：P70266

Rat Gene Id：24638

Rat Swiss Prot No：P07953

Immunogen：The antiserum was produced against synthesized peptide derived from human PFKFB1. AA range:406-455

Specificity：PFK-2 liv Polyclonal Antibody detects endogenous levels of PFK-2 liv protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PFKFB1;F6PK;PFRX;6-phosphofructo-2-kinase/fructose-2;6-bisphosphatase 1;6PF-2-K/Fru-2,6-P2ase 1;PFK/FBPase 1;6PF-2-K/Fru-2,6-P2ase liver isozyme

Observed Band(KD)：58kD

Background： This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012],

Function：catalytic activity:ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.,catalytic activity:Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate.,enzyme regulation:Phosphorylation results in inhibition of the kinase activity.,function:Synthesis and degradation of fructose 2,6-bisphosphate.,similarity:In the C-terminal section; belongs to the phosphoglycerate mutase family.,subunit:Homodimer.,tissue specificity:Liver.,

Subcellular Location：cytosol,6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex,

Expression：Liver.