Target：PERK

Fields：Mitophagy - animal;Autophagy - animal;Protein processing in endoplasmic reticulum;Apoptosis;Non-alcoholic fatty liver disease;Alzheimer disease;Parkinson disease;Amyotrophic lateral sclerosis;Prion disease;Pathways of neurodegeneration - multiple diseases;Hepatitis C;Measles;Herpes simplex virus 1 infection;Lipid and atherosclerosis

Gene Name：EIF2AK3 PEK PERK

Protein Name：Eukaryotic translation initiation factor 2-alpha kinase 3 (EC 2.7.11.1) (PRKR-like endoplasmic reticulum kinase) (Pancreatic eIF2-alpha kinase) (HsPEK)

Human Gene Id：9451

Human Swiss Prot No：Q9NZJ5

Mouse Swiss Prot No：Q9Z2B5

Rat Gene Id：29702

Rat Swiss Prot No：Q9Z1Z1

Immunogen：Synthesized peptide derived from human PEK/PERK Polyclonal

Specificity：This antibody detects endogenous levels of PEK/PERK.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000, ELISA 1:10000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：Eukaryotic translation initiation factor 2-alpha kinase 3 (EC 2.7.11.1) (PRKR-like endoplasmic reticulum kinase) (Pancreatic eIF2-alpha kinase) (HsPEK)

Observed Band(KD)：130kD

Background： The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.,domain:The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.,enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase act

Subcellular Location：Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Expression：Ubiquitous. A high level expression is seen in secretory tissues.