Target：PEDF

Fields：Wnt signaling pathway

Gene Name：SERPINF1

Protein Name：Pigment epithelium-derived factor

Human Gene Id：5176

Human Swiss Prot No：P36955

Mouse Gene Id：20317

Mouse Swiss Prot No：P97298

Immunogen：The antiserum was produced against synthesized peptide derived from human PEDF. AA range:258-307

Specificity：PEDF Polyclonal Antibody detects endogenous levels of PEDF protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：SERPINF1;PEDF;PIG35;Pigment epithelium-derived factor;PEDF;Cell proliferation-inducing gene 35 protein;EPC-1;Serpin F1

Observed Band(KD)：46kD

Background： This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016],

Function：developmental stage:Expressed in quiescent cells.,domain:The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.,function:Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.,PTM:The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.,similarity:Belongs to the serpin family.,subcellular location:Enriched in stage I melanosomes.,tissue specificity:Retinal pigment epithelial cells and blood plasma.,

Subcellular Location：Secreted . Melanosome . Enriched in stage I melanosomes.

Expression：Retinal pigment epithelial cells and blood plasma.