Target:PHF1
Gene Name:PHF1
Protein Name:PHD finger protein 1
Human Gene Id:5252
Human Swiss Prot No:O43189
Mouse Gene Id:21652
Mouse Swiss Prot No:Q9Z1B8
Immunogen:The antiserum was produced against synthesized peptide derived from human PHF1. AA range:345-394
Specificity:PHF1 Polyclonal Antibody detects endogenous levels of PHF1 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:PHF1;PCL1;PHD finger protein 1;Protein PHF1;Polycomb-like protein 1;hPCl1
Observed Band(KD):62kD
Background: This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
Function:function:Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.,similarity:Contains 2 PHD-type zinc fingers.,subcellular location:Localizes specifically to the promoters of numerous target genes.,subunit:Interacts with CHMP1 (By similarity). Interacts with the PRC2 complex.,tissue specificity:Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.,
Subcellular Location:Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes specifically to the promoters of numerous target genes. Localizes to double-strand breaks (DSBs) sites following DNA damage. Co-localizes with NEK6 in the centrosome.
Expression:Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.