Target：PRP6

Fields：Spliceosome

Gene Name：PRPF6

Protein Name：Pre-mRNA-processing factor 6

Human Gene Id：24148

Human Swiss Prot No：O94906

Mouse Gene Id：68879

Mouse Swiss Prot No：Q91YR7

Rat Gene Id：366276

Rat Swiss Prot No：A1A5S1

Immunogen：The antiserum was produced against synthesized peptide derived from human PRPF6. AA range:747-796

Specificity：PRP6 Polyclonal Antibody detects endogenous levels of PRP6 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PRPF6;C20orf14;Pre-mRNA-processing factor 6;Androgen receptor N-terminal domain-transactivating protein 1;ANT-1;PRP6 homolog;U5 snRNP-associated 102 kDa protein;U5-102 kDa protein

Observed Band(KD)：110kD

Background： The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. [provided by RefSeq, Jul 2008],

Function：function:Involved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation.,similarity:Contains 9 HAT repeats.,subunit:Associates with the U5 snRNP particle (containing the 40 kDa, 52 kDa, 116 kDa, 200 kDa and 220 kDa proteins) probably via interaction(s) with the 220 kDa and/or other proteins. Associates with U4/U6 snRNP particle (containing the 15.5 kDa, 20 kDa/60kDa/90kDa heteromer, LSm proteins LSm2-8, 61 kDa and Sm proteins). Interacts with ARAF1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1

Subcellular Location：Nucleus, nucleoplasm . Nucleus speckle . Localized in splicing speckles. .

Expression：Widely expressed.