Target:PRIM1
Fields:DNA replication
Gene Name:PRIM1
Protein Name:DNA primase small subunit
Human Gene Id:5557
Human Swiss Prot No:P49642
Mouse Gene Id:19075
Mouse Swiss Prot No:P20664
Immunogen:The antiserum was produced against synthesized peptide derived from human PRIM1. AA range:371-420
Specificity:PRIM1 Polyclonal Antibody detects endogenous levels of PRIM1 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:PRIM1;DNA primase small subunit;DNA primase 49 kDa subunit;p49
Observed Band(KD):50kD
Background: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008],
Function:function:DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.,miscellaneous:The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA.,similarity:Belongs to the eukaryotic-type primase small subunit family.,subunit:Heterodimer of a small subunit and a large subunit.,
Subcellular Location:nucleoplasm,alpha DNA polymerase:primase complex,membrane,primosome complex,
Expression: Bone marrow,Thymus,