Target：PUMA

Fields：Platinum drug resistance;p53 signaling pathway;Apoptosis;Apoptosis - multiple species;Hippo signaling pathway;Huntington disease;Measles;Pathways in cancer;Colorectal cancer

Gene Name：BBC3

Protein Name：Bcl-2-binding component 3

Human Gene Id：27113

Human Swiss Prot No：Q9BXH1

Mouse Gene Id：170770

Mouse Swiss Prot No：Q99ML1

Rat Gene Id：317673

Rat Swiss Prot No：Q80ZG6

Immunogen：The antiserum was produced against synthesized peptide derived from human PUMA. AA range:120-169

Specificity：PUMA Polyclonal Antibody detects endogenous levels of PUMA protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：BBC3;PUMA;Bcl-2-binding component 3;JFY-1;p53 up-regulated modulator of apoptosis

Observed Band(KD)：23kD

Background： This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011],

Function：function:Essential mediator of p53-dependent and p53-independent apoptosis.,induction:By DNA damage, glucocorticoid treatment, growth factor deprivation and p53.,similarity:Belongs to the Bcl-2 family.,subcellular location:Localized to the mitochondria in order to induce cytochrome c release.,subunit:Interacts with MCL1 and BCL2A1 (By similarity). Interacts with BCL2 and BCL2L1/BCL-XL.,tissue specificity:Ubiquitously expressed.,

Subcellular Location：Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release.

Expression：Ubiquitously expressed.