Target：Rad18

Gene Name：RAD18

Protein Name：E3 ubiquitin-protein ligase RAD18

Human Gene Id：56852

Human Swiss Prot No：Q9NS91

Mouse Swiss Prot No：Q9QXK2

Immunogen：The antiserum was produced against synthesized peptide derived from human RAD18. AA range:301-350

Specificity：Rad18 Polyclonal Antibody detects endogenous levels of Rad18 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：RAD18;RNF73;E3 ubiquitin-protein ligase RAD18;Postreplication repair protein RAD18;hHR18;hRAD18;RING finger protein 73

Observed Band(KD)：56kD

Background： The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008],

Function：function:E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the RAD18 family.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 SAP domain.,subunit:Interacts with UBE2A and UBE2B. Interacts with SHPRH.,

Subcellular Location：Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565). .

Expression： Epithelium,Liver,Placenta,