Target:RAB8A
Fields:Autophagy - animal;Endocytosis;AMPK signaling pathway;Tight junction;Pancreatic secretion;Amyotrophic lateral sclerosis;Pathways of neurodegeneration - multiple diseases
Gene Name:RAB8A MEL RAB8
Protein Name:Ras-related protein Rab-8A (Oncogene c-mel)
Human Gene Id:4218
Human Swiss Prot No:P61006
Mouse Swiss Prot No:P55258
Rat Swiss Prot No:P35280
Immunogen:Synthesized peptide derived from human protein . at AA range: 120-200
Specificity:RAB8A Polyclonal Antibody detects endogenous levels of protein.
Formulation:Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Observed Band(KD):22kD
Background: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malign
Function:function:May be involved in vesicular trafficking and neurotransmitter release.,similarity:Belongs to the small GTPase superfamily. Rab family.,subunit:Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3.,
Subcellular Location:Cell membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus . Recycling endosome membrane . Cell projection, cilium . Cytoplasmic vesicle, phagosome . Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasm, cytoskeleton, cilium basal body . Midbody . Cytoplasm . Cytoplasm, cytoskeleton, cilium axoneme . Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (PubMed:15837803). In the GDP-bound form, present in the perinuclear region (PubMed:12221131). Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form (PubMed:12221131). Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical me
Expression: Brain,Epithelium,Platelet,Skin,