Target:RECK
Fields:MicroRNAs in cancer
Gene Name:RECK
Protein Name:Reversion-inducing cysteine-rich protein with Kazal motifs
Human Gene Id:8434
Human Swiss Prot No:O95980
Mouse Gene Id:53614
Mouse Swiss Prot No:Q9Z0J1
Immunogen:The antiserum was produced against synthesized peptide derived from human RECK. AA range:21-70
Specificity:RECK Polyclonal Antibody detects endogenous levels of RECK protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:RECK;ST15;Reversion-inducing cysteine-rich protein with Kazal motifs;hRECK;Suppressor of tumorigenicity 15 protein
Observed Band(KD):110kD
Background: The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015],
Function:function:Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.,PTM:N-glycosylated.,similarity:Contains 3 Kazal-like domains.,subunit:Interacts with MMP-9.,tissue specificity:Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.,
Subcellular Location:Cell membrane ; Lipid-anchor, GPI-anchor .
Expression:Expressed in various tissues and untransformed cells (PubMed:9789069). It is undetectable in tumor-derived cell lines and oncogenically transformed cells (PubMed:9789069).