Target：TCF-3

Fields：Signaling pathways regulating pluripotency of stem cells;Human T-cell leukemia virus 1 infection;Transcriptional misregulation in cancer

Gene Name：TCF3

Protein Name：Transcription factor E2-alpha

Human Gene Id：6929

Human Swiss Prot No：P15923

Mouse Gene Id：21423

Mouse Swiss Prot No：P15806

Rat Gene Id：171046

Rat Swiss Prot No：P21677

Immunogen：The antiserum was produced against synthesized peptide derived from human TCF3. AA range:571-620

Specificity：TCF-3 Polyclonal Antibody detects endogenous levels of TCF-3 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TCF3;BHLHB21;E2A;ITF1;Transcription factor E2-alpha;Class B basic helix-loop-helix protein 21;bHLHb21;Immunoglobulin enhancer-binding factor E12/E47;Immunoglobulin transcription factor 1;Kappa-E2-binding factor;Transcription facto

Observed Band(KD)：68kD

Background： This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have bee

Function：disease:Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.,function:Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.,PTM:Phosphorylated following NGF stimulation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with ASH1 and TWIST2. Isoform E12 interacts with GRIPE and FIGLA (By similarity). Interacts with PTF1A and TGFB1I1.

Subcellular Location：Nucleus .

Expression： Lymphoma,Muscle,PCR rescued clones,