Target：TFIIIB90-1

Gene Name：BRF1

Protein Name：Transcription factor IIIB 90 kDa subunit

Human Gene Id：2972

Human Swiss Prot No：Q92994

Mouse Swiss Prot No：Q8CFK2

Immunogen：The antiserum was produced against synthesized peptide derived from human TF3B. AA range:231-280

Specificity：TFIIIB90-1 Polyclonal Antibody detects endogenous levels of TFIIIB90-1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：BRF1;BRF;GTF3B;TAF3B2;TAF3C;Transcription factor IIIB 90 kDa subunit;TFIIIB90;hTFIIIB90;B-related factor 1;BRF-1;hBRF;TAF3B2;TATA box-binding protein-associated factor; RNA polymerase III, subunit 2

Observed Band(KD)：74kD

Background： This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011],

Function：function:General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the TFIIB family.,similarity:Contains 1 TFIIB-type zinc finger.,subunit:TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1.,

Subcellular Location：Nucleus .

Expression： Brain,Epithelium,PCR rescued clones