Target:U2AF1
Fields:Spliceosome;Shigellosis
Gene Name:U2AF1 U2AF35 U2AFBP FP793
Protein Name:Splicing factor U2AF 35 kDa subunit (U2 auxiliary factor 35 kDa subunit) (U2 small nuclear RNA auxiliary factor 1) (U2 snRNP auxiliary factor small subunit)
Human Gene Id:7307
Human Swiss Prot No:Q01081
Mouse Swiss Prot No:Q9D883
Immunogen:Synthesized peptide derived from human protein . at AA range: 60-140
Specificity:U2AF1 Polyclonal Antibody detects endogenous levels of protein.
Formulation:Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Observed Band(KD):26kD
Background: This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
Function:domain:The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.,function:Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 1 RRM (RNA recognition motif) domain.,similarity:Contains 2 C3H1-type zinc fingers.,subunit:Identified in the spliceosome C
Subcellular Location:Nucleus . Nucleus speckle .
Expression: B-cell lymphoma,Bone marrow,Epithelium,Fetal brain,Lung,Prostate,Sp