Target：TNKS1

Gene Name：TNKS PARP5A PARPL TIN1 TINF1 TNKS1

Protein Name：Tankyrase-1 (TANK1) (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 5) (ARTD5) (Poly [ADP-ribose] polymerase 5A) (TNKS-1) (TRF1-interacting ankyrin-related ADP-ribose polymerase) (Tankyras

Human Gene Id：8658

Human Swiss Prot No：O95271

Mouse Swiss Prot No：Q6PFX9

Immunogen：Synthesized peptide derived from part region of human protein

Specificity：TNKS1 Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：145kD

Background：catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.,PTM:ADP-ribosylated (-auto).,PTM:Phosphorylated on serine residues by MAPK kinases upon insulin stimulation.,similarity:Contains 1 PARP catalytic domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 15 ANK repeats.,subcellular location:Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.,subunit:Oligomerizes and associates with TNKS2. Interacts with the cytoplasmic domain of LNPEP/Otase in SLC2A4/GLUT4-vesicles. Binds to the N-terminus of telomeric TERF1 via the ANK repeats. Found in a complex with POT1; TERF1 and TINF2.,tissue specificity:Ubiquitous; highest levels in testis.,

Function：catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.,PTM:ADP-ribosylated (-auto).,PTM:Phosphorylated on serine residues by MAPK kinases upon insulin stimulation.,similarity:Contains 1 PARP catalytic domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 15 ANK repeats.,subcellular location:Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.,subunit:Oligomerizes and associates

Subcellular Location：Cytoplasm . Golgi apparatus membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus, nuclear pore complex . Chromosome, telomere . Cytoplasm, cytoskeleton, spindle pole . Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles (PubMed:22864114). A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres (PubMed:10523501). During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1 (PubMed:12768206). Localizes to spindle poles at mitosis onset via interaction with NUMA1 (PubMed:12080061). .

Expression：Ubiquitous; highest levels in testis.