Target:V-ATPase D
Fields:Oxidative phosphorylation;Metabolic pathways;Phagosome;mTOR signaling pathway;Synaptic vesicle cycle;Collecting duct acid secretion;Vibrio cholerae infection;Epithelial cell signaling in Helicobacter pylori infection;Human papillomavirus infection;Rheumatoid arthritis
Gene Name:ATP6V1D
Protein Name:V-type proton ATPase subunit D
Human Gene Id:51382
Human Swiss Prot No:Q9Y5K8
Mouse Gene Id:73834
Mouse Swiss Prot No:P57746
Immunogen:Synthesized peptide derived from V-ATPase D . at AA range: 70-150
Specificity:V-ATPase D Polyclonal Antibody detects endogenous levels of V-ATPase D protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:ATP6V1D;ATP6M;VATD;V-type proton ATPase subunit D;V-ATPase subunit D;V-ATPase 28 kDa accessory protein;Vacuolar proton pump subunit D
Observed Band(KD):28kD
Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008],
Function:function:Subunit of the peripheral V1 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.,similarity:Belongs to the V-ATPase D subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).,
Subcellular Location:Membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cell projection, cilium . Localizes to centrosome and the base of the cilium. .
Expression: Bone marrow,Brain,Heart,Pancreatic adenocarcinoma,Pituitary,Placent