Target：WEE1

Fields：Cell cycle;Human immunodeficiency virus 1 infection

Gene Name：WEE1

Protein Name：Wee1-like protein kinase

Human Gene Id：7465

Human Swiss Prot No：P30291

Mouse Gene Id：22390

Mouse Swiss Prot No：P47810

Rat Gene Id：308937

Rat Swiss Prot No：Q63802

Immunogen：The antiserum was produced against synthesized peptide derived from human WEE1. AA range:19-68

Specificity：Wee 1 Polyclonal Antibody detects endogenous levels of Wee 1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：WEE1;Wee1-like protein kinase;WEE1hu;Wee1A kinase

Observed Band(KD)：72kD

Background：WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 2 magnesium ions per subunit.,enzyme regulation:Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.,function:May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase

Subcellular Location：Nucleus.

Expression： Amygdala,Blood,Epithelium,Human uterus endothel primary cell culture,Placenta,Skin,