Target：XRCC1

Fields：Base excision repair

Gene Name：XRCC1

Protein Name：DNA repair protein XRCC1

Human Gene Id：7515

Human Swiss Prot No：P18887

Mouse Gene Id：22594

Mouse Swiss Prot No：Q60596

Rat Gene Id：84495

Rat Swiss Prot No：Q9ESZ0

Immunogen：The antiserum was produced against synthesized peptide derived from human XRCC1. AA range:517-566

Specificity：XRCC1 Polyclonal Antibody detects endogenous levels of XRCC1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：XRCC1;DNA repair protein XRCC1;X-ray repair cross-complementing protein 1

Observed Band(KD)：70kD

Background： The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008],

Function：function:Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.,polymorphism:Carriers of the polymorphic Gln-399 allele may be at greater risk for tobacco- and age-related DNA damage.,PTM:Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.,PTM:Sumoylated.,similarity:Contains 2 BRCT domains.,subcellular location:Accumulates at sites of DNA damage.,subunit:Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF.,

Subcellular Location：Nucleus . Moves from the nucleoli to the global nuclear chromatin upon DNA damage. .

Expression：Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).