Target:Repo-Man
Gene Name:CDCA2
Protein Name:Cell division cycle-associated protein 2
Human Gene Id:157313
Human Swiss Prot No:Q69YH5
Mouse Swiss Prot No:Q14B71
Immunogen:The antiserum was produced against synthesized peptide derived from human CDCA2. AA range:511-560
Specificity:Repo-Man Polyclonal Antibody detects endogenous levels of Repo-Man protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:CDCA2;Cell division cycle-associated protein 2;Recruits PP1 onto mitotic chromatin at anaphase protein;Repo-Man
Observed Band(KD):120-130kD
Background:cell division cycle associated 2(CDCA2) Homo sapiens This gene encodes a targeting subunit of the cell-cycle associated protein, protein phosphatase 1, with a role in targeting this protein to chromatin during anaphase. These two proteins comprise a phosphatase complex that is involved in nuclear envelope reformation and regulation of the DNA damage response. The encoded protein may also play a role in cancer progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015],
Function:function:Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.,PTM:Phosphorylated by CDK1. May regulate its subcellular location.,subcellular location:Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin.,subunit:Interacts with PPP1CC.,tissue specificity:Ubiquitously expressed.,
Subcellular Location:Nucleus . Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin.
Expression:Ubiquitously expressed.