Target：Repac

Fields：Ras signaling pathway;Rap1 signaling pathway

Gene Name：RAPGEF5

Protein Name：Rap guanine nucleotide exchange factor 5

Human Gene Id：9771

Human Swiss Prot No：Q92565

Mouse Gene Id：217944

Mouse Swiss Prot No：Q8C0Q9

Rat Gene Id：362799

Rat Swiss Prot No：P83900

Immunogen：The antiserum was produced against synthesized peptide derived from human RAPGEF5. AA range:431-480

Specificity：Repac Polyclonal Antibody detects endogenous levels of Repac protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：RAPGEF5;GFR;KIAA0277;MRGEF;Rap guanine nucleotide exchange factor 5;Guanine nucleotide exchange factor for Rap1;M-Ras-regulated Rap GEF;MR-GEF;Related to Epac;Repac

Observed Band(KD)：60kD

Background： Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008],

Function：function:Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,tissue specificity:Widely expressed with highest levels in brain.,

Subcellular Location：Nucleus.

Expression：Widely expressed with highest levels in brain.