Target:ZO3
Fields:Tight junction
Gene Name:TJP3 ZO3
Protein Name:Tight junction protein ZO-3 (Tight junction protein 3) (Zona occludens protein 3) (Zonula occludens protein 3)
Human Swiss Prot No:O95049
Mouse Swiss Prot No:Q9QXY1
Immunogen:Synthesized peptide derived from part region of human protein
Specificity:ZO3 Polyclonal Antibody detects endogenous levels of protein.
Formulation:Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Observed Band(KD):102kD
Background: The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012],
Function:similarity:Belongs to the MAGUK family.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 3 PDZ (DHR) domains.,subunit:Interacts with occludin, claudins and ZO-1. Interacts with INADL (By similarity). Interacts with UBN1.,
Subcellular Location:Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction . Nucleus . Exhibits predominant nuclear expression in proliferating cells but is exclusively junctionally expressed after confluence is reached (PubMed:23608536). Shows an epithelial-specific tight junction localization in a TJP1/TJP2-dependent fashion (By similarity). .
Expression: PCR rescued clones,Whole embryo,