Target：17β-HSD4

Fields：Primary bile acid biosynthesis;Biosynthesis of unsaturated fatty acids;Metabolic pathways;Fatty acid metabolism;Peroxisome

Gene Name：HSD17B4

Protein Name：Peroxisomal multifunctional enzyme type 2

Human Gene Id：3295

Human Swiss Prot No：P51659

Mouse Gene Id：15488

Mouse Swiss Prot No：P51660

Rat Gene Id：79244

Rat Swiss Prot No：P97852

Immunogen：The antiserum was produced against synthesized peptide derived from the N-terminal region of human HSD17B4. AA range:41-90

Specificity：17β-HSD4 Polyclonal Antibody detects endogenous levels of 17β-HSD4 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：HSD17B4;EDH17B4;Peroxisomal multifunctional enzyme type 2;MFE-2;17-beta-hydroxysteroid dehydrogenase 4;17-beta-HSD 4;D-bifunctional protein;DBP;Multifunctional protein 2;MPF-2

Observed Band(KD)：80kD

Background：hydroxysteroid 17-beta dehydrogenase 4(HSD17B4) Homo sapiens The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014],

Function：catalytic activity:(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl-CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA + H(2)O.,catalytic activity:(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.,disease:Defects in HSD17B4 are a cause of D-bifunctional protein deficiency (DBPD) [MIM:261515]. DBPD is a disorder of peroxisomal fatty acid beta-oxidation.,function:Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.,pathway:Lipid metabolism; fatty acid beta-oxidation.,similarity:Belongs to the short-chain dehydrogenases/reductases (SDR) family.,similarity:Contains 1 SCP2 domain.,tissue specificity:Present in many tissues with highest concentrations in liver, heart, prostate and testis.,

Subcellular Location：Peroxisome .

Expression：Present in many tissues with highest concentrations in liver, heart, prostate and testis.