Target：IF5A1

Gene Name：EIF5A

Protein Name：Eukaryotic translation initiation factor 5A-1 (eIF-5A-1) (eIF-5A1) (Eukaryotic initiation factor 5A isoform 1) (eIF-5A) (Rev-binding factor) (eIF-4D)

Human Gene Id：1984

Human Swiss Prot No：P63241

Mouse Swiss Prot No：P63242

Rat Swiss Prot No：Q3T1J1

Immunogen：Synthesized peptide derived from part region of human protein

Specificity：IF5A1 Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：16kD

Background：function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.,tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,

Function：function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.,tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,

Subcellular Location：Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). .

Expression：Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).