Target：IBTK

Gene Name：IBTK BTKI KIAA1417

Protein Name：Inhibitor of Bruton tyrosine kinase (IBtk)

Human Gene Id：25998

Human Swiss Prot No：Q9P2D0

Mouse Swiss Prot No：Q6ZPR6

Immunogen：Synthesized peptide derived from human protein . at AA range: 410-490

Specificity：IBTK Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：148kD

Background： Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014],

Function：function:Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.,similarity:Contains 2 BTB (POZ) domains.,similarity:Contains 3 ANK repeats.,similarity:Contains 3 RCC1 repeats.,subcellular location:Translocates to the plasma membrane upon IgM stimulation.,subunit:Interacts with the PH domain of BTK.,tissue specificity:Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.,

Subcellular Location：Cytoplasm. Membrane; Peripheral membrane protein. Translocates to the plasma membrane upon IgM stimulation.; [Isoform 2]: Nucleus.

Expression：Expressed in DeFew, HEK293T, HeLa and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues.